| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to cellular engineering, biotechnology and food industry. Disclosed is a method of culturing myoblasts in vitro to obtain myoblast biomass, where the cultured cells used are immotalised animal myoblasts; cells are grown using a culture medium with non-animal proteins, and also containing haemoglobin, wherein the cells are kept in a proliferative state using fibroblast and/or hepatocyte growth factors to form biomas, and differentiation of myoblasts into myocytes is then induced by removing fibroblast and/or hepatocyte growth factors from the medium, and in the case of cells with normal myostatin expression, a myostatin inhibitor is added to the medium.EFFECT: method can be used in food industry to produce safe meat food products in large amounts over a short period of time.6 cl, 3 tbl, 9 ex |
