| 摘要 |
FIELD: measurement equipment.SUBSTANCE: invention related to the field of laboratory medical analysis, analytic instrument-making. The method consists in the fact that parameters of conversion for a system of image recording with specified equipment functions, including the main components of normalised spectral signals r(?)=V(?)/V(?), where ?- reference spectral section, and coefficients of non-linear multiple regressions between concentrations of chromophores Cand normalised signals r(?) or their projections to space of main components, they determine by means of simulation of radiation transfer into tissues with account of characteristics of the used system of recording and possible ranges of variations of structural and biochemical parameters of the tissue. At the same time the tissue is illuminated with a linearly polarised radiation, a multispectral image is recorded V(x, y, ?) with polarisation, orthogonal to polarisation of illuminating radiation, additionally they record a multispectral polarised image V(x, y, ?) with absence of tissue illumination. For each point x, y they produce normalised spectral signals r(?) by normalisation of layers of a differential multispectral image V(x, y, ?)-V(x, y, ?) to a differential reference layer V(x, y, ?)-?(x, y, ?), and concentration of chromophores C(x, y) in each point x, y is determined in accordance with corresponding normalised spectral signals using non-linear multiple regressions between Cand r(?) or between Cand projections r(?) to the space of main components.EFFECT: invention provides for increased accuracy of production of 2D distributions of biological tissue chromophore concentration.6 dwg, 1 tbl |
