MOLECULAR MARKER AND ANALYSIS METHOD FOR IDENTIFICTION OF RIVER DRAINAGE SYSTEM OF GOBIOBOTIA MACROCEPHALA

摘要

The present invention relates to a marker for group identifying Gobiobotia macrocephala in Gum river and Gobiobotia macrocephal in Han river, and to a method for group identifying the Gobiobotia macrocephala in Gum river and Gobiobotia macrocephal in Han river using the marker. A SCAR marker for analyzing the Gobiobotia macrocephala according to an embodiment of the present invention includes a primer described in sequence number 5 or 6. In addition, the method for analyzing Gobiobotia macrocephala water system according to the embodiment of the present invention comprises the steps of: isolating genomic DNA of the Gobiobotia macrocephala; amplifying using the isolated genomic DNA as a template by a PCR primer for COI region of mitochondirial DNA of the Gobiobotia macrocephala described in sequence number 1 or 2; performing electrophorosis for the amplified products; and identifying as the Gobiobotia macrocephala in Kum river if two or more of the amplified products are existed, and as the Gobiobotia macrocephala in Han river if one of the amplifed product is existed. The marker used in the present invention is rapid, simple and has high accuracy so that efficiency and practicality can be maximized when discharged and the marker can be used as a marker for dividing into two groups when discharging or mixing Gobiobotia macrocephala which are in a crisis of extinction.