发明名称 |
FUNCTIONALISATION METHODS AND REAGENTS USED IN SUCH METHODS USING AN AZAISATOIC ANHYDRIDE OR ONE OF THE DERIVATIVES OF SAME, BIOLOGICAL MOLECULES THEREBY TREATED, AND KITS |
摘要 |
<p>The present invention concerns a method for functionalising at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which comprises the following steps: a) providing at least: - one binding molecule constituted by an azaisatoic anhydride or one of the derivatives of same, - a moiety of interest, and - a binding arm connecting the binding molecule with the moiety of interest, b) causing the anhydride function of the binding molecule to react with at least one hydroxyl moiety carried: - in position 2' of the ribose of one of the RNA nucleotides, and/or - in position(s) 2' and/or 3' of the ribose of the nucleotide at terminal end 3' of the RNA, and c) obtaining an aza-anthranylate that binds the RNA to the moiety of interest via the binding arm.</p> |
申请公布号 |
WO2014019966(A1) |
申请公布日期 |
2014.02.06 |
申请号 |
WO2013EP65854 |
申请日期 |
2013.07.26 |
申请人 |
BIOMERIEUX |
发明人 |
CAILLY, THOMAS;FABIS, FREDERIC;LAAYOUN, ALI;LAURENT, ALAIN;URSUEGUI, SYLVAIN |
分类号 |
C07D213/80;C07D495/04;C07D519/00;C07H21/00;C12Q1/68 |
主分类号 |
C07D213/80 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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