ASSAY

摘要

The present invention provides a method of determining the presence of an analyte in a liquid sample. The method comprises contacting a first binding moiety with the liquid sample, the first binding moiety being linked to signal moiety which yields a signal and specifically binding the analyte to form a reaction conjugate. A detection conjugate is formed by binding the reaction conjugate to a reducing agent-generating moiety. The detection conjugate is contacted with a substrate for the reducing agent-generating moiety and metal ions; and the signal yielded by the signal moiety is measured. A change in the signal indicates the presence of the analyte.