| 摘要 |
<p>The present invention relates to a method for nucleic acid amplification, and detection or sequencing, comprising the steps of, a. providing a double stranded target nucleic acid molecule for amplification, b. terminally attaching to said target nucleic acid molecule both up-stream and downstream a first and second linker nucleic acid comprising a protelomerase target sequence, c. reacting said target nucleic acid molecule with a protelomerase, preferably as encoded by SEQ ID NO. 1, such that the target nucleic acid is converted to a circular target nucleic acid, d. amplifying the circular target nucleic acid in an amplification reaction comprising a polymerase and at least one first primer which binds outside the protelomerase target sequence such that an in parts self-complementary amplification product is synthesized.</p> |
