| 摘要 |
The present invention provides a method for enriching target sequences from a sequencing library to provide a target enriched sequencing library, wherein the sequencing library is suitable for massive parallel sequencing and comprises a plurality of double-stranded nucleic acid molecules, wherein the method comprises: a) providing nucleoprotein filaments comprising (i) a single stranded invasion probe, wherein the invasion probe has a region of substantial complementarity to one strand of a double-stranded target sequence, (ii) a recombinase; b) forming a complex between the invasion probe and a complementary portion of the target sequence wherein complex formation is mediated by the recombinase; c) separating the complexes from the remaining sequencing library, thereby enriching the target sequences. The present invention relies on the use of a recombinase such as Rec A for enriching target nucleic acid sequences from a sequencing library. |
