SCAVENGER CONTAINING PROTEIN FORMED FROM TANDEM-TYPE MULTIMER OF MUTANT EXTRACELLULAR DOMAIN OF PROTEIN G

摘要

The purpose of the present invention is to provide an excellent protein that maintains high antibody binding activity under neutrality but has a lower bindability with the Fc region and/or bindability with the Fab region of immunoglobulin under weak acidity when compared to a protein containing the extracellular domain of wild protein G. Another purpose of the present invention is to provide for the easy capture and recovery of antibody by a scavenger and column that use the protein without denaturing the antibody. The present invention relates to a scavenger of antibody, immunoglobulin G, or a protein having the Fc region of immunoglobulin G, characterized in that a protein formed from a tandem-type multimer of the mutant extracellular domain having binding activity with a protein comprising the Fc region of immunoglobulin G is anchored to a water-soluble solid-phase substrate primarily via amino groups. The present invention also relates to a chromatography column for protein separation and purification packed with the scavenger, and the like.