| 摘要 |
An apparatus and a method are provided for column chromatography, comprising a cylindrical chromatography column 15, the column having an inlet and an outlet 411. The inlet is configured to introduce a flow of mobile phase 2 into the column carrying a sample, whereby the sample separates into components as it advances longitudinally through the column from the inlet to the outlet carried by the mobile phase, wherein the inlet is further configured to introduce the flow of mobile phase into the column in at least two separate portions which are independently controllable, and to introduce the portions into different radial regions 440, 442 of the column, such that the portions flow longitudinally through the column in different radial regions, and wherein the mobile phase leaves the column as a flow of eluate and the outlet is configured to split the flow of eluate as it leaves the column into at least two separate eluate portions from the different radial regions of the column and the apparatus is configured to separately process the eluate portions. |
