PHOTOMETRIC ANALYSIS DEVICE, PHOTOMETRIC ANALYSIS METHOD, AND COMPUTER PROGRAM FOR PHOTOMETRIC ANALYSIS, USING SINGLE LIGHT-EMITTING PARTICLE DETECTION

摘要

There is provided a structure to make the setting of a criterion for eliminating noises easy in the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, time series light intensity data of light from a light detection region detected with moving the position of the light detection region in the sample solution by changing the optical path of the optical system of the microscope is generated, and a signal of a light-emitting particle individually is detected in the time series light intensity data. In detecting the signal of the light-emitting particle, a signal having a light intensity in a light intensity range set based upon a signal generation frequency integrated value distribution which is a distribution, obtained by using as a variable an intensity of a signal, of integrated values of generation frequencies of signals having an intensity not lower than the variable is extracted as the signal of the light-emitting particle.