发明名称 METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CARDIOMYOCYTES
摘要 The object is to provide a method for differentiation induction of a myocardial cell from a stem cell in a selective manner with high efficiency. Disclosed is a method for differentiation induction of a myocardial cell from a pluripotent stem cell, comprising the following steps (i) and (ii): (i) culturing the pluripotent stem cell in a culture medium containing no substance capable of promoting the activation of the canonical Wnt signaling pathway, wherein the culturing is started at the point of time at which the differentiation induction is initiated and is ended 24 hours before the expression of canonical Wnt gene starts to increase; and (ii) culturing the pluripotent stem cell in a culture medium containing a substance capable of promoting the activation of the canonical Wnt signaling pathway, wherein the culturing is started 24 to 0 hour before the expression of the canonical Wnt gene starts to increase and is continued for 24 to 96 hours.
申请公布号 CA2650685(C) 申请公布日期 2014.02.04
申请号 CA20072650685 申请日期 2007.04.27
申请人 ASUBIO PHARMA CO., LTD. 发明人 KOSHIMIZU, UICHI;TANAKA, TOMOFUMI;KAWASHIMA, KAYOKO;KADOKURA, MICHINORI
分类号 C12N5/00;C12N5/0735;C12N5/077 主分类号 C12N5/00
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