BLOOD FLOW ADJUSTMENT FOR MAXIMIZING NUCLEATED CELL RECOVERY

摘要

Provided is a method for improving the recovery of nucleated cells of the final product in order to use human umbilical cord blood for hematopoietic stem cell transplantation or stem cell transplantation. More particularly, the present invention relates to a method of adjusting the blood flow rate in a bag after centrifugation and controlling an outlet in order to effectively separate the nucleated cells corresponding to a flow layer between the plasma and the red blood cells, in the procedure of removing the plasma and red blood cells and concentrate nucleated cells for the cryopreservation or study of the cord blood added with an anticoagulant through manipulation. Since the umbilical cord blood containing a large number of nucleated cells per unit is recognized as having high quality, the method of the present invention capable of obtaining high-quality nucleated cells from the limited umbilical cord blood as a biological resource can be immediately used by the cord blood bank currently operating. [Reference numerals] (AA) Blood flow rate control; (BB) Bag position change; (CC) Bag fixing device on the scale