Diagnostic devices and related methods

摘要

596163 Disclosed is a test strip configured to receive a sample for detection of an analyte therein, the test strip comprising: a substrate; a coating on a portion of the substrate, the coating comprising a combination of a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte; and a first analyte binding agent and a second analyte binding agent that are each labeled with a detectable marker, wherein the first analyte binding agent is labeled with a first fluorophore and the second analyte binding agent is labeled with a second fluorophore that is different from the first fluorophore, and wherein the first fluorophore and the second fluorophore fluoresce upon application of light of wavelengths from about 300 nm to about 800 nm. Further disclosed is a method for detecting at least one analyte in a sample comprising: applying the sample to a portion of a test strip comprising a coating comprising a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte; and applying light of wavelengths from about 300 nm to about 800 nm to the test strip, wherein the application of light to the test strip provides an indication of whether the first analyte is present in the sample.