| 摘要 |
602848 Disclosed is a method of increasing viable cell density in a fed batch eukaryotic cell culture comprising the steps of: a) culturing a Chinese Hamster Ovary (CHO) cell line selected from CHO-Kl and CHO-K1SV, wherein said cell line expresses both of the heterologous apoptotic-resistant (apoptoticR) genes E1B19K and AVEN and one or more genes of interest; and b) maintaining a high glucose media feed, containing about 60mM glucose or greater, during the exponential and stationary phases of the cell culture. |
