发明名称 |
MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS |
摘要 |
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule. |
申请公布号 |
US2014030725(A1) |
申请公布日期 |
2014.01.30 |
申请号 |
US201213674655 |
申请日期 |
2012.11.12 |
申请人 |
PROMEGA CORPORATION;PROMEGA CORPORATION |
发明人 |
BINKOWSKI BROCK;BUTLER BRAEDEN;ENCELL LANCE P.;FAN FRANK;HOOK BRAD;OTTO PAUL;RISMA KIMBERLY;VIDUGIRIS GEDIMINAS;WIGDAL SUSAN;ZIMMERMAN KRISTOPHER |
分类号 |
C12Q1/66;C12N9/02 |
主分类号 |
C12Q1/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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