<p>Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.</p>
申请公布号
WO2014018828(A1)
申请公布日期
2014.01.30
申请号
WO2013US52189
申请日期
2013.07.26
申请人
NEW ENGLAND BIOLABS, INC.;TANNER, NATHAN;ZHANG, YINHUA;EVANS, THOMAS, C.