发明名称 |
Methods of transcription activator like effector assembly |
摘要 |
The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets. |
申请公布号 |
AU2012284365(A1) |
申请公布日期 |
2014.01.30 |
申请号 |
AU20120284365 |
申请日期 |
2012.07.12 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
JOUNG, J. KEITH;SANDER, JEFFRY D. |
分类号 |
C12N15/66;C07K14/24;C07K19/00;C12N1/00;C12N5/00;C12N15/31;C12N15/62;C12Q1/68;C40B40/06 |
主分类号 |
C12N15/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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