发明名称 |
Non-fluorescent quencher compounds and biomolecular assays |
摘要 |
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection. |
申请公布号 |
US8637658(B2) |
申请公布日期 |
2014.01.28 |
申请号 |
US201213629021 |
申请日期 |
2012.09.27 |
申请人 |
EWING-FIERI DEBRA KAY;APPLIED BIOSYSTEMS, LLC |
发明人 |
EWING GREGORY J.;MULLAH KHAIRUZZAMAN BASHAR;GRAHAM RONALD |
分类号 |
C07H19/04;A61K38/00;C07C245/08;C07C245/10;C07C255/67;C07C317/32;C07D221/18;C07D241/16;C07F9/141;C12Q1/00;C12Q1/68;G01N33/533 |
主分类号 |
C07H19/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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