Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria

摘要

Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 mumol min-1 mg-1. LAB cells with even higher activities of 0.2 to 0.6 mumol min-1 mg-1 of DHAD activity were obtained. These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.