发明名称 |
Methods for increasing product yields |
摘要 |
A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product. |
申请公布号 |
US8637286(B2) |
申请公布日期 |
2014.01.28 |
申请号 |
US201113011818 |
申请日期 |
2011.01.21 |
申请人 |
BURGARD ANTHONY P.;OSTERHOUT ROBIN E.;SUN JUN;PHARKYA PRITI;GENOMATICA, INC. |
发明人 |
BURGARD ANTHONY P.;OSTERHOUT ROBIN E.;SUN JUN;PHARKYA PRITI |
分类号 |
C12N9/00;C12N1/20 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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