| 摘要 |
FIELD: medicine.SUBSTANCE: invention describes a method for polymer immunoglobulin diagnosticum engineering for detecting serogroup 1, 3 and 6 Legionella pneumophila, including preparing rabbit immune serums and sensitising them on a polymer carrier in the form of microspheres for the purpose of detecting the strains L. pneumophila in a slide agglutination test; the antibody polymer carrier is polyacrolein which at the stage of polymerisation is processed in safranin T; the microspheres have the diameter of 1 mcm and contain aldehyde groups 0.4 mcmole/g. Then, the carrier is additionally processed in 5% aqueous tannin at 37-40°C for three hours and kept for 15 hours at room temperature, washed in water by centrifugation until a negative reaction on FeCb and phenols is observed in a supernatant. Thereafter, the microspheres are sensitised with the rabbit Legionella serum; the carrier 25 mg is suspended in physiological solution 0.9 ml and added with the diluted (1:40) serum 0.1 ml; the prepared suspension is agitated for 2 hours at room temperature, then for 15 hour at 4°C, washed in sodium chloride and suspended in the same solution 1 ml with 1% gelatose; the prepared diagnosticum is preserved and poured out in 5 ml containers for use and storage.EFFECT: method provides detecting the high-specific, ostentation and low-cost agent of serougroup 1, 3 and 6 Legionella pneumophila.5 cl, 3 ex, 3 tbl |
