The kit for detection of reproductive toxicants using toxicity of endocrine disrupting chemicals and the method for diagnosis thereof

摘要

PURPOSE: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor and a kit for detecting reproductive toxicant are provided to identify reproductive toxicity mechanism and to study ecotoxicogenomics. CONSTITUTION: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor contains a biomarker. The biomarker is an oligonucleotide containing 15-50 nucleic acids or complementary strand molecule thereof. A method for detecting the biomarker to identify reproductive toxicity induced by endocrine disruptor comprises: a step of synthesizing cDNA from RNA samples isolated from regenerative cells of an experimental group(male individual which is suspicious for endocrine disruptor exposure) and a control group; a step of labeling the cDNA with different fluorescent substances; a step of hybridizing the cDNA with the DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing gene expression level of the DNA microarray chip between the experimental group and control group. [Reference numerals] (AA) Reproduction; (BB) Cell proliferation; (CC) Signal transduction; (DD) Cell adhesion; (EE) Cell cycle; (FF) Immune response; (GG) Inflammatory response; (HH) Cell apoptosis; (II) Transport; (JJ) Lipid metabolism; (KK) Protein synthesis; (LL) Gene transcription; (MM) Homeostasis; (NN) Cell growth; (OO) Cell differentiation