VECTORS WITH MODIFIED INITIATION CODON FOR TRANSLATION OF AAV-REP78 USEFUL FOR PRODUCTION OF AAV IN INSECT CELLS

摘要

PROBLEM TO BE SOLVED: To provide: nucleic acid constructs for production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells; insect cells comprising such constructs; and methods for using these cells to produce recombinant parvoviral virions.SOLUTION: The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins, and thereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in the insect cells. The insect cells further comprise a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.