SIGNALLING COMPOUNDS FOR USE IN METHODS OF DETECTING HYDROGEN PEROXIDE

摘要

Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of formula
is reacted with peroxide. A detectable product compound of the formula Sig - OH or Sig-O is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. The signaling compound has the formula:
wherein B is a boron atom, R 5 and R 6 are independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring or an arylene ring, and R 13 is independently selected from cyano, imine, carbonyl, thiazole, carbonyl-substitute thiazole and benzothiazole groups or a group
wherein Z is C-C double or triple bond or aromatic ring and n is 1 or 2.