Coupled enzyme systems for determination of dissolved substances

摘要

A test system having an extended range of measurement and a corresponding procedure for the determination of substances in liquids contains several enzymes or enzyme systems together which, independently of one another, react directly or indirectly with the substance to be determined. On adding the test system to the sample solution, various final products are produced which can be distinguished analytically and evaluated by measurement techniques or visually. For example, glucose can be determined over a wide range of concentrations using a combination of an NAD-dependent dehydrogenase and a non-NAD-dependent dehydrogenase.