发明名称 |
Nucleotide transient binding for sequencing methods |
摘要 |
Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation. |
申请公布号 |
US8632975(B2) |
申请公布日期 |
2014.01.21 |
申请号 |
US20100790760 |
申请日期 |
2010.05.28 |
申请人 |
VANDER HORN PETER B.;CHEN CHENG-YAO;LUO GUOBIN;PREVITE MICHAEL;TEMIROV JAMSHID;NIKIFOROV THEO;ZHOU ZHAOHUI;SUN HONGYE;WANG YUFANG;NISHIMURA STEFANIE YUKIKO;WANG HONGYI;PERIS MARIAN;ROSENBLUM BARNETT B.;PHELAN MICHAEL;LIFE TECHNOLOGIES CORPORATION |
发明人 |
VANDER HORN PETER B.;CHEN CHENG-YAO;LUO GUOBIN;PREVITE MICHAEL;TEMIROV JAMSHID;NIKIFOROV THEO;ZHOU ZHAOHUI;SUN HONGYE;WANG YUFANG;NISHIMURA STEFANIE YUKIKO;WANG HONGYI;PERIS MARIAN;ROSENBLUM BARNETT B.;PHELAN MICHAEL |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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