METHOD FOR VITAL IMMOBILISATION OF HUMAN AND ANIMAL SPERM CELLS

摘要

FIELD: medicine.SUBSTANCE: what is presented is medium and method for the vital immobilisation of human and animal sperm cells, wherein the sperm cells of native or thawed sperm prepared by the ejaculate centrifugation in a density gradient, or sampled by the flotation; the sperm cells are transferred into an immobilisation medium with low osmotic pressure 100-170 mOsm/kg for 1 min to 2 hours with the gas mixture composition containing 0.1 to 8% of carbon dioxide, and a medium temperature of 0 to 39°C.EFFECT: invention may be used for diagnostic and therapeutic purposes for animal reproduction and fertilisation.7 cl, 1 tbl