| 摘要 |
PURPOSE: A manufacturing method of a carbon nanotube substrate for regulating the growth and differentiation of cells is provided to regulate the growth and differentiation of Human mesenchymal stem cells (hMSCs) separated from bone marrow without a differentiation medium by spin-coating carbon nanotubes on a substrate in order to control the density of the carbon nanotubes on the substrate. CONSTITUTION: A manufacturing method of a carbon nanotube substrate for regulating the growth and differentiation of cells comprises the steps of: aligning carbon nanotubes on a substrate in one direction based on a spin-coating method; and coating poly (ethylene glycol) (PEG) on the substrate with aligned carbon nanotubes. The substrate is a glass cover coated with titanium (Ti) and gold (Au). The carbon nanotubes are single-walled carbon nanotubes (SWNT). The rotation speed of the spin-coating process is in the range of 4000-6000 rpm. During the carbon nanotube aligning step, the surface density of the aligned carbon nanotubes is controlled on the substrate in order to regulate the growth and differentiation of hMSCs. A method for regulating the growth and differentiation of hMSCs comprises a step of culturing hMSCs separated from bone marrow on a carbon nanotube substrate. [Reference numerals] (AA) Example 1; (BB) Comparative example 1 |
