NOVEL MONOCLONAL ANTIBODY THAT SPECIFICALLY BINDS TO DLL4 AND USE THEREOF

摘要

The present invention relates to a novel monoclonal antibody specifically binding to delta like ligand 4 (DLL4) and, more specifically, a monoclonal antibody specifically binding to human DLL4 and effectively inhibiting the binding between the DLL4 and Notch receptor, polynucleotide coding the monoclonal antibody, an expression vector including the polynucleotide, a transformant including the expression vector, a method for producing the monoclonal antibody, a pharmaceutical composition containing the monoclonal antibody for preventing or treating cancers, a composition containing the monoclonal antibody for diagnosis of the cancers, a diagnosis method using the monoclonal antibody, and a pharmaceutical composition containing the monoclonal antibody for preventing or treating autoimmune diseases. [Reference numerals] (AA) VH sequence; (BB,HH) Clone name; (C1) MLCK-1 (Sequence number 1); (C2) MLCK-2 (Sequence number 5); (C3) MLCK-3 (Sequence number 8); (C4) MLCK-4 (Sequence number 11); (D1,D2,D3) GFTFSDYAMS (Sequence number 2); (D4) GFTFSDYAMS (Sequence number 12); (E1) WTYSDDGNKYYADSVKG (Sequence number 3); (E2) WTYSGSGNKYYADSVKG (Sequence number 6); (E3) WTYYDSGSKYYADSVKG (Sequence number 9); (E4) WTYHGGGDTYYADSVKG (Sequence number 13); (F1) ADPPFDY (Sequence number 4); (F2) ADWPFDY (Sequence number 7); (F3) ADLPFDY (Sequence number 10); (F4) GPNYTFGKPFDY (Sequence number 14); (GG) VL sequence; (I1) MLCK-1 (Sequence number 15); (I2) MLCK-2 (Sequence number 19); (I3) MLCK-3 (Sequence number 23); (I4) MLCK-4 (Sequence number 27); (J1) TGSSSNIGSNNVS (Sequence number 16); (J2) TGSSSNIGSNDVT (Sequence number 20); (J3) SGSSSNIGNNAVT (Sequence number 24); (J4) RGSPSNIGNNTVY (Sequence number 28); (K1) SDNNRPS (Sequence number 17); (K2) ADSKRPS (Sequence number 21); (K3) SDNHRPS (Sequence number 25); (K4) SDSQRPS (Sequence number 29); (L1) ATWDSSLNGYV (Sequence number 18); (L2) GTWDYSLSAYV (Sequence number 22); (L3) GTWDASLSGYV (Sequence number 26); (L4) GSWDYSLSAYV (Sequence number 30)