发明名称 |
METHOD FOR INHIBITING NUCLEIC ACID AMPLIFICATION USING LIGHT AND HIGHLY SENSITIVE METHOD FOR SELECTIVE NUCLEIC ACID AMPLIFICATION |
摘要 |
<p>Provided is a method for rapidly and easily detecting a mutated nucleic acid, which is contained in a small amount in a nucleic acid sample together with wild-type nucleic acids, with high specificity and high sensitivity. In the method of the present invention, amplification of a detection region comprising a target site by a nucleic acid amplification method is inhibited, by the steps of allowing a nucleic acid having a target site to coexist with a clamp probe comprising a photo-crosslinking nucleic acid and having a sequence complementary to the target site, and photo-crosslinking the nucleic acid having the target site with the clamp probe by photo-irradiation.</p> |
申请公布号 |
EP2615171(A4) |
申请公布日期 |
2014.01.15 |
申请号 |
EP20110823664 |
申请日期 |
2011.09.09 |
申请人 |
MITSUBISHI CHEMICAL MEDIENCE CORPORATION |
发明人 |
TERASAKI HIROSHI;KONNO TSUNETADA;SHIMADZU MITSUNOBU;FUJIMOTO KENZO;SAKAMOTO TAKASHI |
分类号 |
C12N15/09;C12M1/00;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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