发明名称 |
NOVEL EXTRACELLULARLY SECRETED NUCLEASE |
摘要 |
The purpose of the present invention is to provide a nuclease that secretes natural nonpathogenic microorganisms extracellularly, has higher specific activity than conventional nucleases, and is useful in nucleolytic degradation on an industrial scale. This purpose is achieved with an extracellularly secreted nuclease derived from Streptomyces bacteria, the nuclease having specific activity equal to or greater than the specific activity of Benzonase ® when supplied to double-stranded DNA for 30 minutes at 37°C in 20 mM Tris/HCl (pH 8.5) containing 1 mM MgCl 2 and 1 mM CaCl 2 after purification, using double-stranded DNA, single-stranded DNA, and RNA as substrates. |
申请公布号 |
EP2617824(A4) |
申请公布日期 |
2014.01.15 |
申请号 |
EP20110825241 |
申请日期 |
2011.09.15 |
申请人 |
INDEPENDENT ADMINISTRATIVE INSTITUTION JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY |
发明人 |
MORI, KOZUE;OHTA, YUKARI;HATADA, YUJI;NAKAMURA, NOBUYUKI;MIYAZAKI, MASAYUKI |
分类号 |
C12N9/22;C12N1/20;C12N15/09;C12Q1/42;C12Q1/68;C12R1/465 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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