| 摘要 |
Primers and probes are provided for selectively detecting tubercle bacillus, and methods for detecting low copy numbers of human tubercle bacillus (Mycobacterium tuberculosis) using the same are presented. Oligonucleotides comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:S, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof are disclosed, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. Primers and probes containing such oligonucleotides, and methods for detecting Mycobacterium tuberculosis using the primers and the probes are presented. |
