DNA-SEQUENCE AND RECOMBINANT PRODUCTION OF GROUP 4 MAJOR ALLERGENS FROM CEREALS

摘要

DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen are new. (I) have sequences (1; 1603 nucleotides (nt)); (3; 1644 nt); (5; 1608 nt); (7; 1603 nt) or (9; 1603 nt), all reproduced. DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen comprise sequences comprising 1603 nucleotides (SEQ ID No.:1) (nt)); 1644 nucleotides (SEQ ID No.:3; ); 1608 nucleotides (SEQ ID No.:5; ); 1603 nucleotides (SEQ ID No.:7; ) or 1603 nucleotides (SEQ ID No.:9; ). Independent claims are also included for: (1) DNA (Ia) that hybridizes to (I) under stringent conditions and is derived from DNA of Poaceae; (2) DNA (Ib) that encodes a polypeptide that is immunologically cross-reactive with the major allergens Sec c 4 ( Secale cereale); Hor v 4 ( Hordeum vulgare) or Tri a 4 ( Triticum aestivum) and derived from Poaceae; (3) DNA fragment, or combination of fragments, of (I), (Ia) or (Ib) that encodes an immunomodulatory T-cell reactive fragment (X) of Group 4 Poaceae allergens; (4) a DNA corresponding to any of the new DNAs or fragments that encodes an (X) but is modified by targeted mutation of individual codons, deletion or addition; (5) recombinant DNA expression vector or cloning system containing any of the new DNAs linked to expression control sequences; (6) host organism transformed with any of the new DNAs or the vector of (5); (7) preparing a polypeptide (II) encoded by the new DNAs by culturing the organisms of (6); and (8) (II) having any of the sequences, comprising 518 amino acids (SEQ ID No.:2; 520 amino acids (SEQ ID No.:4; ); 518 amino acids (SEQ ID No.:6; ); 518 amino acids (SEQ ID No.:8) or 518 amino acids (SEQ ID No.: 10; ) or their mature forms. ACTIVITY : Antiallergic. No biological data given. MECHANISM OF ACTION : Vaccine.