NOVEL EXPRESSION AND SECRETION VECTOR SYSTEMS FOR HETEROLOGOUS PROTEIN PRODUCTION IN ESCHERICHIA COLI

摘要

The present invention relates to a recombinant DNA expression/secretion system in E. coliwherein the said system combines the potential of signal peptide-based translocation of recombinant proteins to the periplasmic space of E. coli with membrane defective mutants of E. coli to further aid secretion into the extracellular space. The present invention further relates to the expression system which furthermore includes a helper plasmid to drive the expression of translocons to facilitate improved periplasmic secretion of the over-expressed recombinant protein. In addition, this system also facilitates efficient production of specific proteins of interest in E. coli.