METHOD FOR ASSESSING CONDITIONS OF PROLIFERATIVE CERVICAL DISEASES

摘要

FIELD: medicine.SUBSTANCE: method is staged: 1) cervical canal scrapes and biopsy materials of the uterine cervix are used to recover DNA of human papilloma virus; 2) virus genotype is determined; 3) patients with HPV 16 positive CIN are sampled; 4) real-time PCR is used to determine the number of virus DNA copies and a degree of its integration with a host gene (a physical status); 5) a threshold level of the viral load is stated taking into account the physical status of the virus (6.5 lg HPV DNA copies 16 per one cell); 6) the patients are classified according to the determined threshold viral load and physical status of the virus (the episomal and integrated form); 7) the poorly predicted patients having the high viral load (?6.5 lg DNA copies per one cell) are detected in the episomal form of the virus, while the low load (<6.5 lg DNA copies per one cell) is shown by the integrated form of the virus.EFFECT: method enables the early prediction of the unfavourable course of cervical intraepithelial neoplasms and potential transition into cervical cancer.3 cl, 2 dwg, 1 tbl, 4 ex