发明名称 |
PROCESS FOR PRODUCTION OF PROTEIN |
摘要 |
This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest. |
申请公布号 |
EP2441831(A4) |
申请公布日期 |
2014.01.08 |
申请号 |
EP20100786229 |
申请日期 |
2010.06.10 |
申请人 |
INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS;KYOWA HAKKO KIRIN CO., LTD. |
发明人 |
KAWAKAMI, KOICHI;YAMAGUCHI, KEINA;OGAWA, RISA;TSUKAHARA, MASAYOSHI |
分类号 |
C12N15/00;C12N5/10;C12N15/09;C12N15/85;C12P21/02;C12P21/08 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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