| 摘要 |
The invention relates to a family of invertebrate genes that are transcribed in the immediate early phase following exposure to Fibroblast Growth Factors (FGF) during mesoderm indu ction, termed Mesoderm Induction Immediate Early Response (MIER) genes. Defining featur es of the members of this family include that these genes are a) transcribed in response t o fibroblast growth factors (FGF); b) are expressed within 40 minutes of FGF treatment; and c ) do not require protein synthesis for transcription. There are at least eleven members w ithin this family; as a description, the cloning and characterization of a cDNA representing a memb er of the MIER family, er1 is presented. The invention relates generally to compositions of and diagnostic methods relati ng to the MIER gene family, cDNA, nucleotide fragments, polypeptides coded thereby, recombinant host cells and vectors containing MIER encoding polynucleotide sequences, recombinant MIER polypeptides, and antibodies. By way of example, the invention discloses the clo ning and functional expression of different MIER polypeptides. The invention also include s methods for using the isolated, recombinant polynucleotides, polypeptides, and antibodies di rected thereto in assays designed to select substances which interact with MIER polypeptides for u se diagnostic and therapeutic applications in addition to drug design and DNA vaccination meth odologies.
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