发明名称 |
EVOLVING NEW MOLECULAR FUNCTION |
摘要 |
Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein. |
申请公布号 |
CA2441820(C) |
申请公布日期 |
2014.01.07 |
申请号 |
CA20022441820 |
申请日期 |
2002.03.19 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
LIU, DAVID R.;GARTNER, ZEV J.;KANAN, MATTEW W. |
分类号 |
C07H21/00;C12N15/09;C07B61/00;C07H21/02;C07H21/04;C08G85/00;C12N15/10;C12P1/00;C12P19/34;C12P21/00;C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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