| 摘要 |
The present invention relates to a kit for screening HPV antibody using a fusion polypeptide HPV antigen. More particularly, the present invention relates to a fusion polypeptide comprising an amino acid sequence of a Histidine tag at the N-terminal, an amino acid sequence of a Simian virus 40 large T-antigen at the C-terminal or its active fragment, and an amino acid sequence of an HPV L1 antigen selected from the group consisting of HPV 6 L1 antigen, HPV11 L1 antigen, HPV16 L1 antigen, and HPV18 L1 antigen, or its active fragment; a method for detecting an HPV antibody in a sample by using the fusion polypeptide HPV antigen; and an HPV antibody detecting kit for a bead array, comprising the fusion polypeptide HPV antigen. The use of the present invention can isolate and purify a recombinant HPV antigen at high purity without influencing activity to specifically bind to the HPV antibody and attach the recombinant HPV antigen to beads for a bead array effectively and uniformly, so that HPV 6, 11, 16, and 18 antibodies can be complexly detected in the sample with high sensitivity and accuracy. Further, the present invention can be utilized to determine the inoculation time of HPV vaccine and determine the defense period after inoculation by measuring the antibody titer. |
