TARGETED RNA-SEQ METHODS AND MATERIALS FOR THE DIAGNOSIS OF PROSTATE CANCER

摘要

Methods for diagnosing the presence of a disorder, such as prostate cancer, in a subject are provided, such methods including detecting the relative frequency of expression of RNA biomarkers in a biological sample obtained from the subject using RNA-Seq technology and comparing the relative levels of expression with predetermined threshold levels. Levels of expression of at least two of the RNA biomarkers that are above the predetermined threshold levels are indicative of the presence of prostate cancer in the subject. The methods involve the use of at least one gene-specific primer in at least one point of the RNA-Seq protocol for targeted sequencing of relevant transcripts.