发明名称 |
MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS |
摘要 |
A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Ban virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity. |
申请公布号 |
US2014004123(A1) |
申请公布日期 |
2014.01.02 |
申请号 |
US201313870804 |
申请日期 |
2013.04.25 |
申请人 |
INSTITUTE FOR RESEARCH IN BIOMEDICINE |
发明人 |
LANZAVECCHIA ANTONIO |
分类号 |
C07K16/10;A61K39/395;A61P31/14;C07K16/00;C07K16/12;C07K16/20;C12N5/0781;C12N5/24;C12P21/00;C12P21/08;C12Q;G01N33/49 |
主分类号 |
C07K16/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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