发明名称 |
Recombinant peptide production using a cross-linkable solubility tag |
摘要 |
The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest. |
申请公布号 |
US8617843(B2) |
申请公布日期 |
2013.12.31 |
申请号 |
US201113080917 |
申请日期 |
2011.04.06 |
申请人 |
ALSOP ALBERT W.;CHENG QIONG;SOLOMON LINDA JANE;FAHNESTOCK STEPHEN R.;GRUBER TANJA MARIA;ROUVIERE PIERRE E.;E I DU PONT DE NEMOURS AND COMPANY |
发明人 |
ALSOP ALBERT W.;CHENG QIONG;SOLOMON LINDA JANE;FAHNESTOCK STEPHEN R.;GRUBER TANJA MARIA;ROUVIERE PIERRE E. |
分类号 |
C12P21/06;C07K14/00 |
主分类号 |
C12P21/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|