Methods for determining the retention of peptides in reverse phase chromatography using linear solvent strength theory

摘要

The present disclosure relates to methods for separating or isolating a peptide using reverse phase chromatography. The disclosure also relates to methods for calculating or determining the slope S of a peptide, wherein S is defined according to the Linear-Solvent-Strength equation log k=log k0-S*phi. Also provided are a set of peptides with known S values suitable for use in the described methods.