| 摘要 |
<p>In vitro process for obtaining soil bacterial biomass comprises: (i) contacting a soil sample with a matrix substrate to form an area on the surface of the substrate similar to that of the clay-humus complex (AHC); (ii) maturing the area in oligotrophic conditions allowing the cells constituting AHC that have colonized the matrix substrate optionally migrating towards the liquid supernatant phase; (iii) recovering and growing most prolific bacterial strains in liquid rich culture medium, having native, hardy and persistent in situ characters. In vitro process for obtaining soil bacterial biomass comprises: (i) contacting a soil sample with a matrix substrate to form an area on the surface of the substrate similar to that of the clay-humus complex (AHC) for retaining on the surface or in the matrix substrate, mainly of endogenous bacterial microflora in the soil medium of origin; (ii) maturing the area in oligotrophic conditions allowing the cells constituting AHC that have colonized the matrix substrate optionally migrating towards the liquid supernatant phase; and (iii) recovering and growing most prolific bacterial strains in liquid rich culture medium, having native, hardy and persistent in situ characters, where the matrix substrate is carbon and semi-solid, preferably constituted of a non-bactericidal cellulose matrix impregnated with sterile and demineralized water.</p> |
