发明名称 |
AN EXPRESSION CONSTRUCT FOR SENSING CELL DENSITY AND SUBSTRATE AVAILABILITY AND ITS USE IN CONVERSION OF HYDROXYCINNAMIC ACIDS |
摘要 |
An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene encoding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of using the product generated by the first enzyme as a substrate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is inducible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the absence of the product generated by the first enzyme, wherein the third gene is operably linked to a third promoter that regulates expression of the third gene; and its use in producing a product such as hydroxybenzaldehyde. |
申请公布号 |
WO2013191651(A1) |
申请公布日期 |
2013.12.27 |
申请号 |
WO2013SG00242 |
申请日期 |
2013.06.12 |
申请人 |
NANYANG TECHNOLOGICAL UNIVERSITY |
发明人 |
CHANG, MATTHEW WOOK;LO, TAT MING SAMUEL;POH, CHUEH LOO |
分类号 |
C12N15/63;C12N15/70 |
主分类号 |
C12N15/63 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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