发明名称 |
APPARATUS AND METHOD FOR QUANTIFICATION OF REPLICATIVE LIFESPAN AND OBSERVATION OF SENESCENE |
摘要 |
<p>The present invention teaches an apparatus and method for the automated study of senescence of cells. The apparatus includes a plurality of plates, customized with a flow-through hole, arranged on a platform. A microscope with integrated camera is positioned above the platform and both microscope and platform are coupled to a rotation mechanism to allow relative rotation between the platform and the microscope. Cells are anchored magnetically, chemically, or electrostatically to the plates and are treated to a controlled environment. Daughter cells born from the mother cell are observed by the microscope and then washed, using a wash fluid, through the flow-through hole in the plate. A processor automates the process and allows for a user to input customizable test parameters and value thresholds to indicate a test is completed. The processor also organises test data in the form of a spreadsheet for simple modelling and data manipulation.</p> |
申请公布号 |
WO2013188978(A1) |
申请公布日期 |
2013.12.27 |
申请号 |
WO2013CA50474 |
申请日期 |
2013.06.20 |
申请人 |
CYTOSPAN TECHNOLOGIES CORPORATION |
发明人 |
PETTIGREW, MICHAEL JAMES;PETTIGREW, CHRISTOPHER DOUGLAS |
分类号 |
C12Q1/02;C12M1/34;G01N1/34 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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