发明名称 |
IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES |
摘要 |
Acyl-CoA:lysophosphatidylcholine acyltransferase ["LPCAT"] having the ability to convert acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine to CoA + 1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase ["PDAT"] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids ["PUFAs"]. |
申请公布号 |
WO2013192002(A1) |
申请公布日期 |
2013.12.27 |
申请号 |
WO2013US45592 |
申请日期 |
2013.06.13 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
BOSTICK, MICHAEL, W.;YADAV, NARENDRA, S.;ZHANG, HONGXIANG;ZHU, QUINN, QUN |
分类号 |
C12N9/10;C12P7/64 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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地址 |
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