STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN

摘要

FIELD: biotechnologies.SUBSTANCE: recombinant bacteria strain Escherichia coli All-Russian collection of industrial microorganisms B-11271 - producer of hydrolase of esters of alpha-amino acids from Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915 is built, which is obtained by transformation of strain-recipient Escherichia coli BL21(DE3) of plasmid DNA corresponding to nucleotide sequence SEQ ID NO 1 containing gene aehR of hydrolase of alpha-amino acids esters from Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915, which is not modified on 5'-end with the sequence coding a polyhistidin tail and being under control of promoter T7, as well as resistance gene to kanamycin Kan.EFFECT: invention allows obtaining hydrolase of esters of alpha-amino acids with high efficiency degree.2 cl, 3 dwg, 1 tbl, 7 ex