| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a T-cell receptor β-chain and α-chain, specifically recognizing MAGE1 135-143(NYKHCFPEI), MAGE3 195-203(IMPKAGLLI), MART1 27-35(AAGIGILTV), gp100 209-217(IMDQVPFSV), CMVpp65 341-349(QYDPVAALF) or CMVpp65 495-503(NLVPMVATV) which are melanoma-specific peptides, and to provide genes thereof. <P>SOLUTION: The gene classification of expression TCR is carried out by constructing a method for inducing a CTT cell specific to the peptides based on a dendritic cell, purifying the CTL cell by an Auto-MACS method, extracting an RNA from the purified cell, carrying out cDNA synthesis by reverse transcription reaction, and carrying out TCR repertoire analysis by using a PCR method, and the TCR gene is cloned based on the analysis result. The new T-cell receptor β-chain and α-chain specifically recognizing the peptides are identified by analyzing the DNA sequence of a cloned fragment. <P>COPYRIGHT: (C)2009,JPO&INPIT |
