| 摘要 |
The present invention aims to provide a focal position determining method, a focal position determining apparatus, and the like that can determine the focal position of the objective lens focused on an observed target region at the time of setting a specimen, when a specific region in a specimen is defined as the observed target region, and the luminescence of the observed target region is observed. In the present invention, light is irradiated to the specimen, the focal position of the objective lens is changed, the changed focal position is measured, the specimen to which the light is irradiated is imaged at the changed focal position, feature data is calculated based on the imaged image, the process of changing the focal position, the process of measuring the focal position, the process of imaging the specimen, and the process of calculating the feature data are repeatedly executed, at least one focal position is selected from the accumulated plural focal positions by the execution based on the plural feature data accumulated by the execution, and the focal position of the objective lens focused on the observed target region in the specimen is determined based on the selected focal position. The focal position of the objective lens is determined, and then, a digital zooming and optical zooming are performed to magnify the imaged image with a desired magnification for display. |
